Effects of Nicotine on Human Luteal Cells In Vitro: A Possible Role on Reproductive Outcome for Smoking Women.
Miceli F, Minici F, Tropea A, Catino S, Orlando M, Lamanna G, Sagnella F, Tiberi
F, Bompiani A, Mancuso S, Lanzone A, Apa R.
Biol Reprod 72, 628-632. 2005.
We investigated the effect of nicotine and its methylated metabolite,
N-methyl-nicotine (M-nicotine), on human luteal cells by measuring
progesterone and prostaglandins (PGs) release from cultured cells
and by testing Vascular Endothelial Growth Factor (VEGF)gene expression,
an angiogenic factor strictlyinvolved in luteal pathophysiology. Primary
cultures of human luteal cells were treated for 24 hours (h) with
nicotine and M-nicotine (from 10(-6) to 10(-11) M) alone or combined
with hCG 25 ng/ml; progesterone and PGs were assayed in the culture
medium. In another group of experiments luteal cells were treated
for 24 h with nicotine and M-nicotine (10(-7) M) in order to perform
Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) on VEGF mRNA.
Nicotine and M-nicotine negatively affected basal luteal steroidogenesis
at all tested